rabbit polyclonal ab  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SRC , Cell Signaling Technology , Cat# 2109S; RRID: AB_2106059.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Curcumin suppresses metastasis, invasion, and proliferation in osteosarcoma cells by regulating the EGFR/Src signaling axis

doi: 10.1177/03946320241308082

Figure Lengend Snippet: Cur inhibits metastasis of OS dependent on EGFR/Src pathway. (A) In MG63 cells, EGFR intracellular location under Cur treatment or not, Bar=20 um; (B) Construction and identification of Ctrl, Src wild type (SRC-WT)and persistently active plasmid (Src-CA), Bar=20 um; (C) colocation of Exogenous p-Src (Tyr416) and Rab7-positive vesicles after Cur treatment or not in MG63 cells, Bar= 20 um; (D) Relative expression of protein level (PI3K, Akt1, p-Akt1, STAT3, ERK1/2, p-Src, Src, EGFR) in treat with Ctrl, Cur (10 μM), Over-expression of Src-CA and Cur (10 μM) + Over-expression of Src-CA; (E-K) Relative quantification of protein level in Fig 7D.

Article Snippet: Rabbit polyclonal antibodies p-Src (Tyr416) (#6943) were obtained from Cell Signaling Technology (Shanghai, China). p-PI3K (ab182651) were purchased from Abcam (Cambridge, USA).

Techniques: Plasmid Preparation, Expressing, Over Expression, Quantitative Proteomics